Hotspot

rpl16

LSC · 80980-82507 · 1528 bp

Back to Kernera

Pi 0.00131

MarkerSeek score 59.40

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1528
variable_sites: 2
pi: 0.00131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGCAAAGGACGAAATCAAAA	ATTTCCATCAAAACTCCTTTG	35.0	33.3	54.3	52.5	1664 bp	1.0	40.1
rpl16_p2	TGCAAAGGACGAAATCAAAA	AATTTCCATCAAAACTCCTTTG	35.0	31.8	54.3	53.4	1665 bp	1.0	40.1
rpl16_p3	TGCAAAGGACGAAATCAAAAA	ATTTCCATCAAAACTCCTTTG	33.3	33.3	55.2	52.5	1664 bp	1.0	40.1
rpl16_p4	AATGCAAAGGACGAAATCAA	ATTTCCATCAAAACTCCTTTG	35.0	33.3	54.1	52.5	1666 bp	1.0	40.1
rpl16_p5	ATGCAAAGGACGAAATCAAA	ATTTCCATCAAAACTCCTTTG	35.0	33.3	54.1	52.5	1665 bp	1.0	40.1