Hotspot

atpI

LSC · 14198-14947 · 750 bp

Back to Kernera

Pi 0.00133

MarkerSeek score 59.50

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 750
variable_sites: 1
pi: 0.00133
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	TGGGCTAACCTAAAAACGAA	TCTTATTTCTGTCAGAGGGCA	40.0	42.9	54.9	56.4	812 bp	1.0	40.1
atpI_p2	TTGGGCTAACCTAAAAACGA	TCTTATTTCTGTCAGAGGGCA	40.0	42.9	54.9	56.4	813 bp	1.0	40.1
atpI_p3	TTGGGCTAACCTAAAAACGAA	TCTTATTTCTGTCAGAGGGCA	38.1	42.9	55.7	56.4	813 bp	1.0	40.1
atpI_p4	TGGGCTAACCTAAAAACGAA	TCTTATTTCTGTCAGAGGGCAA	40.0	40.9	54.9	57.1	812 bp	1.0	40.1
atpI_p5	TGGGCTAACCTAAAAACGAA	TTCTTATTTCTGTCAGAGGGCA	40.0	40.9	54.9	57.1	813 bp	1.0	40.1