Hotspot

accD

LSC · 57125-58594 · 1470 bp

Back to Kernera

Pi 0.00068

MarkerSeek score 59.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1470
variable_sites: 1
pi: 0.00068
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	AAATAGAGGCCAAAAGCTCT	TCTTGTTGATAGAGGTTTACTGAGT	40.0	36.0	54.6	57.5	1600 bp	1.0	40.1
accD_p2	TGTAAATAGAGGCCAAAAGCTCT	TGTTGATAGAGGTTTACTGAGT	39.1	36.4	57.8	54.2	1600 bp	1.0	40.1
accD_p3	TGTAAATAGAGGCCAAAAGCTCT	TCTTGTTGATAGAGGTTTACTGAGT	39.1	36.0	57.8	57.5	1603 bp	1.0	40.0
accD_p4	TGTAAATAGAGGCCAAAAGCTCT	CTTGTTGATAGAGGTTTACTGAGT	39.1	37.5	57.8	56.2	1602 bp	1.0	40.0
accD_p5	TGTAAATAGAGGCCAAAAGCTC	TCTTGTTGATAGAGGTTTACTGAGT	40.9	36.0	56.4	57.5	1603 bp	1.0	40.0