Hotspot

ndhC-trnV-GUA

LSC · 50278-51178 · 901 bp

Back to Kernera

Pi 0.00111
MarkerSeek score 59.40
Primer available yes
Label ndhC-trnV-GUA

Feature Metadata

label_name
ndhC-trnV-GUA
feature_type
igs
region
LSC
length_bp
901
variable_sites
1
pi
0.00111
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-GUA_p1 TTTCTCTGGCCCCTTCCTAA GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 1042 bp 1.0 74.2
ndhC-trnV-GUA_p2 TCTCTGGCCCCTTCCTAATT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.7 58.7 1040 bp 1.0 72.3
ndhC-trnV-GUA_p3 TTCTCTGGCCCCTTCCTAAT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.7 58.7 1041 bp 1.0 72.3
ndhC-trnV-GUA_p4 TTTCTCTGGCCCCTTCCTAA TTCGAGTCCGTATAGCCCTA 50.0 50.0 57.9 57.1 1041 bp 1.0 72.2
ndhC-trnV-GUA_p5 CTCTGGCCCCTTCCTAATTG GTTCGAGTCCGTATAGCCCT 55.0 55.0 57.6 58.7 1039 bp 1.0 71.8