Hotspot

trnT-ACA-trnL-UUA

LSC · 46367-47168 · 802 bp

Back to Kernera

Pi 0.00125
MarkerSeek score 59.50
Primer available yes
Label trnT-ACA-trnL-UUA

Feature Metadata

label_name
trnT-ACA-trnL-UUA
feature_type
igs
region
LSC
length_bp
802
variable_sites
1
pi
0.00125
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-ACA-trnL-UUA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCAATTCC 55.0 55.0 57.9 58.2 863 bp 1.0 77.8
trnT-ACA-trnL-UUA_p2 TATGGAAAAGCCGGCTATCG CCGTAGCGTCTACCAATTCC 50.0 55.0 57.8 58.2 914 bp 1.0 77.6
trnT-ACA-trnL-UUA_p3 GCGATGCTCTAACCTCTGAG AGTCCGTAGCGTCTACCAAT 55.0 50.0 57.9 58.2 866 bp 1.0 77.1
trnT-ACA-trnL-UUA_p4 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCAATTCC 50.0 55.0 57.8 58.2 895 bp 1.0 77.0
trnT-ACA-trnL-UUA_p5 TATGGAAAAGCCGGCTATCG AGTCCGTAGCGTCTACCAAT 50.0 50.0 57.8 58.2 917 bp 1.0 76.9