Hotspot

trnT-ACC-psbD

LSC · 31572-32823 · 1252 bp

Back to Kernera

Pi 0.0008

MarkerSeek score 59.40

Primer available yes

Label trnT-ACC-psbD

Feature Metadata

label_name: trnT-ACC-psbD
feature_type: igs
region: LSC
length_bp: 1252
variable_sites: 1
pi: 0.0008
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-ACC-psbD_p1	GTGGTAGAGTAACGCCATGG	ACCTACAAAAACGAAGCGGT	55.0	45.0	58.1	58.0	1406-1410 bp	1.0	79.3
trnT-ACC-psbD_p2	TCAGTGGTAGAGTAACGCCA	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1409-1413 bp	1.0	79.1
trnT-ACC-psbD_p3	CCAAGACGGCCCTTTTAACT	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1428-1432 bp	1.0	79.1
trnT-ACC-psbD_p4	CTTAAACCAAGACGGCCCTT	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1434-1438 bp	1.0	79.1
trnT-ACC-psbD_p5	ACGGCCCTTTTAACTCAGTG	ACCTACAAAAACGAAGCGGT	50.0	45.0	58.1	58.0	1423-1427 bp	1.0	79.0