Hotspot

atpH-atpI

LSC · 13698-14197 · 500 bp

Back to Kernera

Pi 0.002

MarkerSeek score 48.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 500
variable_sites: 1
pi: 0.002
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	TACCTTGACCAACTCCAGGT	TTTTTGCCACTTTAGCTGCG	50.0	45.0	57.9	57.9	644 bp	1.0	78.2
atpH-atpI_p2	GTCCAATAGAAGCAAGCCCA	TTTTTGCCACTTTAGCTGCG	50.0	45.0	57.9	57.9	626 bp	1.0	78.0
atpH-atpI_p3	GCTGTACCTTGACCAACTCC	TTTTTGCCACTTTAGCTGCG	55.0	45.0	58.2	57.9	648 bp	1.0	77.5
atpH-atpI_p4	AGGTCCAATAGAAGCAAGCC	TTTTTGCCACTTTAGCTGCG	50.0	45.0	57.6	57.9	628 bp	1.0	75.7
atpH-atpI_p5	GTACCTTGACCAACTCCAGG	TTTTTGCCACTTTAGCTGCG	55.0	45.0	57.5	57.9	645 bp	1.0	75.3