Hotspot

rpl32-trnL-UAG

SSC · 104872-105572 · 701 bp

Back to Kengyilia

Pi 0.00484

MarkerSeek score 57.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 701
variable_sites: 10
pi: 0.00484
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TTCCAGCGGTAATGAGCATC	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.0	58.0	840-847 bp	1.0	80.6
rpl32-trnL-UAG_p2	TTCCAGCGGTAATGAGCATC	GGATTCGAACCGAGATGCTT	50.0	50.0	58.0	58.1	843-850 bp	1.0	80.2
rpl32-trnL-UAG_p3	AAGGGTTTTTCTGGGCAACA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	812-819 bp	1.0	80.0
rpl32-trnL-UAG_p4	AGGGTTTTTCTGGGCAACAA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.1	58.0	811-818 bp	1.0	80.0
rpl32-trnL-UAG_p5	TTCCAGCGGTAATGAGCATC	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	813-820 bp	1.0	79.7