Hotspot

ndhF-rpl32

SSC · 103770-104679 · 910 bp

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Pi 0.00534

MarkerSeek score 46.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 910
variable_sites: 13
pi: 0.00534
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TCCAAATATTTTTACGAATACGCT	55.0	29.2	58.1	55.3	1019-1026 bp	1.0	41.3
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TTGACATTGAAGTACGTTTTT	55.0	28.6	58.1	52.2	992-999 bp	1.0	41.3
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	TTTGACATTGAAGTACGTTTT	55.0	28.6	58.1	52.2	993-1000 bp	1.0	41.3
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TTTTGACATTGAAGTACGTTT	55.0	28.6	58.1	52.2	994-1001 bp	1.0	41.3
ndhF-rpl32_p5	GGAAGTGGGAGAAGAGGGAT	TTTTTGACATTGAAGTACGTT	55.0	28.6	58.1	52.2	995-1002 bp	1.0	41.3