Hotspot

rps8-rpl14

LSC · 77176-77320 · 145 bp

Back to Kengyilia

Pi 0.00397

MarkerSeek score 59.60

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 145
variable_sites: 1
pi: 0.00397
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TCAGCAATAGTGTCCTTGCC	CGATTGCCGAGGAATTGAGA	50.0	50.0	57.9	58.1	226-236 bp	1.0	82.4
rps8-rpl14_p2	GCAATAGTGTCCTTGCCCAT	CGATTGCCGAGGAATTGAGA	50.0	50.0	58.2	58.1	223-233 bp	1.0	81.5
rps8-rpl14_p3	TCAGCAATAGTGTCCTTGCC	GGCAATCCAAAAGGAACTCG	50.0	50.0	57.9	57.4	257-267 bp	1.0	77.4
rps8-rpl14_p4	GCAATAGTGTCCTTGCCCAT	GGCAATCCAAAAGGAACTCG	50.0	50.0	58.2	57.4	254-264 bp	1.0	76.5
rps8-rpl14_p5	TCAGCAATAGTGTCCTTGCC	AGGAACTCGAGTTTTTGGCG	50.0	50.0	57.9	58.8	246-256 bp	1.0	76.4