Hotspot

petA-psbJ

LSC · 60866-61686 · 821 bp

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Pi 0.00183

MarkerSeek score 46.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 4
pi: 0.00183
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GCTGGTATTGCTGTGATTGG	50.0	50.0	58.0	57.1	1015-1016 bp	1.0	73.1
petA-psbJ_p2	GGCCTTTTGTTCTTCTTCGC	GCTGGTATTGCTGTGATTGG	50.0	50.0	57.9	57.1	998-999 bp	1.0	73.0
petA-psbJ_p3	TGTTCTTCTTCGCATCCGTT	GCTGGTATTGCTGTGATTGG	45.0	50.0	57.8	57.1	991-992 bp	1.0	72.2
petA-psbJ_p4	TTGTTCTTCTTCGCATCCGT	GCTGGTATTGCTGTGATTGG	45.0	50.0	57.8	57.1	992-993 bp	1.0	72.2
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	GCTGGTATTGCTGTGATTGG	50.0	50.0	58.2	57.1	1012-1013 bp	1.0	72.0