Hotspot

rbcL-psaI

LSC · 55873-57515 · 1643 bp

Back to Kengyilia

Pi 0.00167

MarkerSeek score 38.50

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1643
variable_sites: 7
pi: 0.00167
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	CCGAAATCTTTGGGGACGAT	AACTCGCTATACCTGGCTCT	50.0	50.0	58.0	57.9	2159-2196 bp	1.0	79.6
rbcL-psaI_p2	TCTTCACATTCACCGTGCAA	AACTCGCTATACCTGGCTCT	45.0	50.0	58.0	57.9	2461-2498 bp	1.0	79.6
rbcL-psaI_p3	TTCTTCACATTCACCGTGCA	AACTCGCTATACCTGGCTCT	45.0	50.0	58.0	57.9	2462-2499 bp	1.0	79.6
rbcL-psaI_p4	CCGAAATCTTTGGGGACGAT	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.0	57.9	1973-2010 bp	1.0	79.5
rbcL-psaI_p5	CTTAACTGGGGGATTCACCG	AACTCGCTATACCTGGCTCT	55.0	50.0	58.0	57.9	2525-2561 bp	0.875	74.5