Hotspot

trnR-UCU-trnfM-CAU

LSC · 35823-35985 · 163 bp

Back to Kengyilia

Pi 0.00321

MarkerSeek score 47.40

Primer available yes

Label trnR-UCU-trnfM-CAU

Feature Metadata

label_name: trnR-UCU-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 163
variable_sites: 2
pi: 0.00321
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnfM-CAU_p1	AGAAGACCTCTGTCCTATCCA	GGCGTATCCAACCCTCTTTT	47.6	50.0	56.9	57.9	302-309 bp	1.0	65.0
trnR-UCU-trnfM-CAU_p2	AGGTTTAGAAGACCTCTGTCCT	GGCGTATCCAACCCTCTTTT	45.5	50.0	57.9	57.9	308-315 bp	1.0	64.3
trnR-UCU-trnfM-CAU_p3	AGAAGACCTCTGTCCTATCCA	AAGGCTCATAACCTTGGGGT	47.6	50.0	56.9	58.6	228-235 bp	1.0	61.8
trnR-UCU-trnfM-CAU_p4	AGAAGACCTCTGTCCTATCCAT	GGCGTATCCAACCCTCTTTT	45.5	50.0	57.4	57.9	302-309 bp	1.0	61.0
trnR-UCU-trnfM-CAU_p5	GGTTTAGAAGACCTCTGTCCT	GGCGTATCCAACCCTCTTTT	47.6	50.0	56.4	57.9	307-314 bp	1.0	60.4