Hotspot

trnT-GGU-trnE-UUC

LSC · 14785-15248 · 464 bp

Back to Kengyilia

Pi 0.00462

MarkerSeek score 37.40

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 464
variable_sites: 6
pi: 0.00462
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	AGTGGTAGAGTAATCTCGTGC	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	56.9	58.3	567-568 bp	1.0	62.3
trnT-GGU-trnE-UUC_p2	GTGGTAGAGTAATCTCGTGCT	GCTGCCTCCTTGAAAGAGAG	47.6	55.0	56.9	58.3	566-567 bp	1.0	62.3
trnT-GGU-trnE-UUC_p3	GTGGTAGAGTAATCTCGTGCT	TGTCCTGAACCACTAGACGA	47.6	50.0	56.9	57.7	545-546 bp	1.0	62.2
trnT-GGU-trnE-UUC_p4	AGTGGTAGAGTAATCTCGTGC	TGTCCTGAACCACTAGACGA	47.6	50.0	56.9	57.7	546-547 bp	1.0	62.1
trnT-GGU-trnE-UUC_p5	GAGTAATCTCGTGCTAAGACGT	GCTGCCTCCTTGAAAGAGAG	45.5	55.0	57.7	58.3	560-561 bp	1.0	60.5