Hotspot

rbcL

LSC · 58709-60136 · 1428 bp

Back to Karpatiosorbus

Pi 0.0007

MarkerSeek score 59.20

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 1
pi: 0.0007
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	CGAGTAGACCTTGTTCTTGTGA	AGTAAAAGATTGGGCCGAGT	45.5	45.0	57.8	56.8	1562 bp	1.0	52.7
rbcL_p2	TCGAGTAGACCTTGTTCTTGTG	AGTAAAAGATTGGGCCGAGT	45.5	45.0	57.8	56.8	1563 bp	1.0	52.7
rbcL_p3	GTCGAGTAGACCTTGTTCTTGT	AGTAAAAGATTGGGCCGAGT	45.5	45.0	57.8	56.8	1564 bp	1.0	52.7
rbcL_p4	CGAGTAGACCTTGTTCTTGTGA	AGTAAAAGATTGGGCCGAGTT	45.5	42.9	57.8	57.6	1562 bp	1.0	50.7
rbcL_p5	TCGAGTAGACCTTGTTCTTGTG	AGTAAAAGATTGGGCCGAGTT	45.5	42.9	57.8	57.6	1563 bp	1.0	50.7