Hotspot

petD

LSC · 80417-81623 · 1207 bp

Back to Karpatiosorbus

Pi 0.000829

MarkerSeek score 59.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1207
variable_sites: 1
pi: 0.000829
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGTTGAAGTGAATTTTCCGAAGA	ACTTTGAAGCGACTATTCCCT	34.8	42.9	56.7	57.0	1311 bp	1.0	40.1
petD_p2	AGTTGAAGTGAATTTTCCGAAGA	CACTTTGAAGCGACTATTCCC	34.8	47.6	56.7	57.0	1312 bp	1.0	40.1
petD_p3	AGTTGAAGTGAATTTTCCGAAGA	GGGAAAATTCACTTTGAAGCGA	34.8	40.9	56.7	57.8	1321 bp	1.0	40.1
petD_p4	AGTTGAAGTGAATTTTCCGAAGA	AGGGAAAATTCACTTTGAAGCG	34.8	40.9	56.7	57.8	1322 bp	1.0	40.1
petD_p5	TGACACTCATAGTTGAAGTGA	ACTTTGAAGCGACTATTCCCT	38.1	42.9	54.3	57.0	1321 bp	1.0	40.1