Hotspot

clpP

LSC · 73783-75852 · 2070 bp

Back to Karpatiosorbus

Pi 0

MarkerSeek score 59.30

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2070
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCTCAAATCACCAATTCGTCTGA	ACGCTTTCACATCAAAGTGA	39.1	40.0	58.1	55.9	2127-2164 bp	1.0	41.4
clpP_p2	CTCAAATCACCAATTCGTCTGA	ACGCTTTCACATCAAAGTGA	40.9	40.0	56.7	55.9	2126-2163 bp	1.0	41.4
clpP_p3	TCTCAAATCACCAATTCGTCTG	ACGCTTTCACATCAAAGTGA	40.9	40.0	56.7	55.9	2127-2164 bp	1.0	41.4
clpP_p4	TCTCAAATCACCAATTCGTCTGA	TCCATTGTTACGCTTTCACA	39.1	40.0	58.1	55.6	2136-2173 bp	1.0	41.4
clpP_p5	TCTCAAATCACCAATTCGTCTGA	CCATTGTTACGCTTTCACATCA	39.1	40.9	58.1	57.6	2135-2172 bp	1.0	41.4