Hotspot

rpl22

LSC · 84109-84702 · 594 bp

Back to Kalidium

Pi 0.0168

MarkerSeek score 53.40

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 594
variable_sites: 10
pi: 0.0168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	AATTTTCGCGCGAATGGTTT	AAGCTTAACACAAAGGCGGA	40.0	45.0	57.9	58.0	1478 bp	1.0	80.3
rpl22_p2	AATTTTCGCGCGAATGGTTT	AGCTTAACACAAAGGCGGAA	40.0	45.0	57.9	58.0	1477 bp	1.0	80.3
rpl22_p3	AATTTTCGCGCGAATGGTTT	TGGGTAGAGCCGGATCTAAG	40.0	55.0	57.9	58.0	1822 bp	1.0	80.2
rpl22_p4	AATTTTCGCGCGAATGGTTT	GGGCCCCAATTGGTAGAAAA	40.0	50.0	57.9	58.1	1723 bp	1.0	79.9
rpl22_p5	GTCCGGCTATAAATTCGGCA	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.1	58.0	1643 bp	0.5	59.0