Hotspot

rps3-rpl22

LSC · 84089-84108 · 20 bp

Back to Kalidium

Pi 0.05

MarkerSeek score 68.10

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 20
variable_sites: 1
pi: 0.05
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	AATTTTCGCGCGAATGGTTT	TACCAGTGGCGTCTACAAGA	40.0	50.0	57.9	58.1	1136 bp	1.0	79.8
rps3-rpl22_p2	AATTTTCGCGCGAATGGTTT	CTTCGTCTATACCAGTGGCG	40.0	55.0	57.9	57.9	1145 bp	1.0	79.5
rps3-rpl22_p3	TGTCCGGCTATAAATTCGGC	TACCAGTGGCGTCTACAAGA	50.0	50.0	58.1	58.1	958 bp	1.0	79.3
rps3-rpl22_p4	AATTTTCGCGCGAATGGTTT	TAAATTTTCGGGGACACGCA	40.0	45.0	57.9	58.1	1306 bp	0.5	58.7
rps3-rpl22_p5	GTCCGGCTATAAATTCGGCA	TACCAGTGGCGTCTACAAGA	50.0	50.0	58.1	58.1	957 bp	0.5	58.3