Hotspot

rps12-clpP

LSC · 70259-70393 · 135 bp

Back to Kalidium

Pi 0.0224

MarkerSeek score 66.10

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 135
variable_sites: 3
pi: 0.0224
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CTAGTACATGTTCCTCGGCG	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.9	57.9	299-300 bp	1.0	79.2
rps12-clpP_p2	CTAGTACATGTTCCTCGGCG	ATGTACAACGAACGGGCAAA	55.0	45.0	57.9	58.4	360-361 bp	1.0	76.7
rps12-clpP_p3	CTAGTACATGTTCCTCGGCG	TATGTACAACGAACGGGCAA	55.0	45.0	57.9	57.5	361-362 bp	1.0	76.3
rps12-clpP_p4	CCTAGTACATGTTCCTCGGC	TATGTCAGCAACAGAAGCCC	55.0	50.0	57.5	57.9	300-301 bp	1.0	76.1
rps12-clpP_p5	CGGGGGATTTCGTGACATTT	TATGTCAGCAACAGAAGCCC	50.0	50.0	58.5	57.9	259-260 bp	1.0	76.0