Hotspot

trnP-UGG-psaJ

LSC · 67157-67586 · 430 bp

Back to Kalidium

Pi 0.0209

MarkerSeek score 67.20

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 430
variable_sites: 9
pi: 0.0209
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	599 bp	1.0	80.2
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	598 bp	1.0	80.2
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	610 bp	1.0	80.1
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	609 bp	1.0	80.1
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	TGTCAACGCATCTGGGAAAA	50.0	45.0	58.1	58.0	612 bp	1.0	80.1