Hotspot

ndhC-trnV-UAC

LSC · 50944-51652 · 709 bp

Back to Kalidium

Pi 0.00871

MarkerSeek score 66.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 6
pi: 0.00871
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTCGGGTCCTTTGTTACTCG	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.0	58.1	860-862 bp	1.0	81.5
ndhC-trnV-UAC_p2	GGTCCTTTGTTACTCGGAGC	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.3	58.1	856-858 bp	1.0	79.4
ndhC-trnV-UAC_p3	CTCGGGTCCTTTGTTACTCG	AGTGCATGTTGGGTCTTTGA	55.0	45.0	58.0	57.6	924-926 bp	1.0	79.3
ndhC-trnV-UAC_p4	GGGTCCTTTGTTACTCGGAG	CCGAGAAAGTCTACGGTTCG	55.0	55.0	57.6	58.1	857-859 bp	1.0	78.5
ndhC-trnV-UAC_p5	GGTCCTTTGTTACTCGGAGC	AGTGCATGTTGGGTCTTTGA	55.0	45.0	58.3	57.6	920-922 bp	1.0	77.1