Hotspot

psaA-ycf3

LSC · 41789-42572 · 784 bp

Back to Kalidium

Pi 0.0128

MarkerSeek score 66.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 10
pi: 0.0128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAAAAATGACCAGGTCGGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	981-1012 bp	1.0	79.7
psaA-ycf3_p2	TGAAAAATGACCAGGTCGGG	ATTGGTTGAAGATCACGGGG	50.0	50.0	58.1	57.9	915-946 bp	1.0	79.1
psaA-ycf3_p3	TGAAAAATGACCAGGTCGGG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	983-1014 bp	1.0	78.9
psaA-ycf3_p4	TGAAAAATGACCAGGTCGGG	AGCCGCTGAGTATTGGAAAC	50.0	50.0	58.1	58.3	977-1008 bp	1.0	78.0
psaA-ycf3_p5	TGAAAAATGACCAGGTCGGG	CAAGCCGCTGAGTATTGGAA	50.0	50.0	58.1	58.3	979-1010 bp	1.0	77.9