Hotspot

trnS-UGA-psbZ

LSC · 35460-35827 · 368 bp

Back to Kalidium

Pi 0.00829

MarkerSeek score 52.40

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 368
variable_sites: 3
pi: 0.00829
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	TACTTGACCAACCATCGGGA	55.0	50.0	58.0	58.4	489-495 bp	1.0	78.5
trnS-UGA-psbZ_p2	GAGCTATCAACCACTCAGCC	TACTTGACCAACCATCGGGA	55.0	50.0	58.1	58.4	496-502 bp	1.0	78.2
trnS-UGA-psbZ_p3	GGAGCTATCAACCACTCAGC	TACTTGACCAACCATCGGGA	55.0	50.0	58.1	58.4	497-503 bp	1.0	78.2
trnS-UGA-psbZ_p4	CAAGACCGGAGCTATCAACC	TACTTGACCAACCATCGGGA	55.0	50.0	58.1	58.4	504-510 bp	1.0	78.1
trnS-UGA-psbZ_p5	CAACCACTCAGCCATCTCTC	CAACCATCGGGAGAAGCAAA	55.0	50.0	58.0	58.5	481-487 bp	1.0	77.6