Hotspot

psbC-trnS-UGA

LSC · 35171-35367 · 197 bp

Back to Kalidium

Pi 0.0355

MarkerSeek score 72.90

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 197
variable_sites: 7
pi: 0.0355
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	GCTGCAGCAGGATTTGAAAA	GAGAGATGGCTGAGTGGTTG	45.0	55.0	57.6	58.0	360-361 bp	1.0	77.8
psbC-trnS-UGA_p2	GCTGCAGCAGGATTTGAAAA	GTTGGAGAGATGGCTGAGTG	45.0	55.0	57.6	58.0	364-365 bp	1.0	77.8
psbC-trnS-UGA_p3	GCTGCAGCAGGATTTGAAAA	GCTGAGTGGTTGATAGCTCC	45.0	55.0	57.6	58.1	352-353 bp	1.0	77.6
psbC-trnS-UGA_p4	GCTGCAGCAGGATTTGAAAA	GGCTGAGTGGTTGATAGCTC	45.0	55.0	57.6	58.1	353-354 bp	1.0	77.6
psbC-trnS-UGA_p5	GCTGCAGCAGGATTTGAAAA	GGTTGATAGCTCCGGTCTTG	45.0	55.0	57.6	58.1	345-346 bp	1.0	77.6