Hotspot

rpoC1-rpoB

LSC · 23575-23600 · 26 bp

Back to Kalidium

Pi 0.0385

MarkerSeek score 64.30

Primer available yes

Label rpoC1-rpoB

Feature Metadata

label_name: rpoC1-rpoB
feature_type: igs
region: LSC
length_bp: 26
variable_sites: 1
pi: 0.0385
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1-rpoB_p1	GCGAAACTGATCCAATTCGG	ACGATCTTTGGCTCTGGAAC	50.0	50.0	57.5	57.9	151 bp	1.0	76.1
rpoC1-rpoB_p2	GGCGAAACTGATCCAATTCG	ACGATCTTTGGCTCTGGAAC	50.0	50.0	57.5	57.9	152 bp	1.0	76.1
rpoC1-rpoB_p3	GCGAAACTGATCCAATTCGG	CCCGAAGATGCTCCAGAATC	50.0	55.0	57.5	58.1	195 bp	1.0	75.8
rpoC1-rpoB_p4	GGCGAAACTGATCCAATTCG	CCCGAAGATGCTCCAGAATC	50.0	55.0	57.5	58.1	196 bp	1.0	75.8
rpoC1-rpoB_p5	TTGAGGCGAAACTGATCCAA	ACGATCTTTGGCTCTGGAAC	45.0	50.0	57.4	57.9	156 bp	1.0	75.2