Hotspot

atpH-atpI

LSC · 13804-14541 · 738 bp

Back to Kalidium

Pi 0.0136

MarkerSeek score 66.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 738
variable_sites: 10
pi: 0.0136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	GCGAATCCATGGAAGGTCAT	45.0	50.0	58.1	58.0	794-801 bp	1.0	80.1
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	GCGAATCCATGGAAGGTCAT	50.0	50.0	57.9	58.0	852-859 bp	1.0	79.9
atpH-atpI_p3	AGCCAATCCAGCAGCAATAA	GCGAATCCATGGAAGGTCAT	45.0	50.0	57.9	58.0	812-819 bp	1.0	79.7
atpH-atpI_p4	CAGCAGCAATAACAGAAGCG	GCGAATCCATGGAAGGTCAT	50.0	50.0	57.8	58.0	804-811 bp	1.0	79.5
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	GCGAATCCATGGAAGGTCAT	55.0	50.0	58.2	58.0	856-863 bp	1.0	79.3