Hotspot

atpF-atpH

LSC · 13109-13557 · 449 bp

Back to Kalidium

Pi 0.0134

MarkerSeek score 63.30

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 449
variable_sites: 6
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGACCCAAGAAAACGA	CCCGAGGCAGAAGGAAAAAT	50.0	50.0	58.0	58.2	611-617 bp	1.0	79.2
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	CCCGAGGCAGAAGGAAAAAT	50.0	50.0	57.9	58.2	638-644 bp	1.0	78.5
atpF-atpH_p3	CCAGTGACCCAAGAAAACGA	CCCGAGGCAGAAGGAAAAATA	50.0	47.6	58.0	58.0	611-617 bp	1.0	72.5
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	CCCGAGGCAGAAGGAAAAATA	50.0	47.6	57.9	58.0	638-644 bp	1.0	71.8
atpF-atpH_p5	GGCCAGTGACCCAAGAAAA	CCCGAGGCAGAAGGAAAAAT	52.6	50.0	57.9	58.2	613-619 bp	1.0	70.5