Hotspot

trnK-UUU-rps16

LSC · 4290-4995 · 706 bp

Back to Kalidium

Pi 0.0115
MarkerSeek score 66.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
706
variable_sites
8
pi
0.0115
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGAAACT 50.0 47.6 58.2 57.9 799-800 bp 1.0 71.5
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGAAACT 50.0 47.6 58.2 57.9 798-799 bp 1.0 71.5
trnK-UUU-rps16_p3 TACCGTTGAGTTAGCAACCC GGTGCTCAACCTACAGAAACT 50.0 47.6 57.5 57.9 785-786 bp 1.0 69.2
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAG GGTGCTCAACCTACAGAAACT 50.0 47.6 58.6 57.9 810-811 bp 1.0 68.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGAAAC 50.0 50.0 58.2 56.4 798-799 bp 1.0 67.5