Hotspot

ycf1

SSC · 120967-126120 · 5154 bp

Back to Kalanchoe

Pi 0.0207

MarkerSeek score 52.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5154
variable_sites: 355
pi: 0.0207
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCTTTTCTTGACCGCATTCC	GGATTCACCGACGAAGATCC	50.0	55.0	58.0	58.1	968-977 bp	1.0	84.5
ycf1_p2	GCTTTTCTTGACCGCATTCC	GGGATTCACCGACGAAGATC	50.0	55.0	58.0	58.1	969-978 bp	1.0	84.5
ycf1_p3	GCTTTTCTTGACCGCATTCC	CAGTAACGGAAGAAAGGGGG	50.0	55.0	58.0	57.9	1060-1069 bp	1.0	84.4
ycf1_p4	GCTTTTCTTGACCGCATTCC	TCACCGACGAAGATCCTTCT	50.0	50.0	58.0	58.2	964-973 bp	1.0	84.4
ycf1_p5	GCTTTTCTTGACCGCATTCC	CAGAATACCGTCATCCGTGG	50.0	55.0	58.0	58.1	1095-1104 bp	1.0	83.9