Hotspot

ccsA-ndhD

SSC · 112565-112817 · 253 bp

Back to Kalanchoe

Pi 0.0699

MarkerSeek score 74.50

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 253
variable_sites: 44
pi: 0.0699
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	AGGGCTACATAGTTATGGTTCA	TCCGTTGATAAGGTTCAAAGT	40.9	38.1	56.3	55.2	324-353 bp	1.0	52.0
ccsA-ndhD_p2	AGGGCTACATAGTTATGGTTCAT	TCCGTTGATAAGGTTCAAAGT	39.1	38.1	56.8	55.2	324-353 bp	1.0	52.0
ccsA-ndhD_p3	GGGCTACATAGTTATGGTTCA	TCCGTTGATAAGGTTCAAAGT	42.9	38.1	54.8	55.2	323-352 bp	1.0	52.0
ccsA-ndhD_p4	AGGGCTACATAGTTATGGTTCA	CCGTTGATAAGGTTCAAAGT	40.9	40.0	56.3	53.5	323-352 bp	1.0	52.0
ccsA-ndhD_p5	AGGGCTACATAGTTATGGTTCA	ATCCGTTGATAAGGTTCAAAGT	40.9	36.4	56.3	55.7	325-354 bp	0.928571	49.1