Hotspot

rpl20-rps12

LSC · 67515-68299 · 785 bp

Back to Kalanchoe

Pi 0.013

MarkerSeek score 64.10

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 785
variable_sites: 38
pi: 0.013
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TTTGTTCTACGTCTCCGAGC	CGCCGAGGAACATGTACTAG	50.0	55.0	57.7	57.9	824-859 bp	1.0	84.0
rpl20-rps12_p2	TGTTCTACGTCTCCGAGCTA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.6	57.9	822-857 bp	1.0	83.6
rpl20-rps12_p3	TTTGTTCTACGTCTCCGAGC	GCTCTTAGGGGATGTCCTCA	50.0	55.0	57.7	58.2	845-880 bp	1.0	83.2
rpl20-rps12_p4	TTTGTTCTACGTCTCCGAGC	TCTTAGGGGATGTCCTCAGC	50.0	55.0	57.7	58.2	843-878 bp	1.0	83.2
rpl20-rps12_p5	TTTGTTCTACGTCTCCGAGC	TACGAAATCCCCCGCTCTTA	50.0	50.0	57.7	58.2	858-893 bp	1.0	83.0