Hotspot

psbM-trnD-GUC

LSC · 29716-30715 · 1000 bp

Back to Kalanchoe

Pi 0.0272

MarkerSeek score 73.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1000
variable_sites: 94
pi: 0.0272
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TCAATTGGTAAGAGCACCGC	45.0	50.0	55.8	58.6	1083-1110 bp	1.0	68.6
psbM-trnD-GUC_p2	AGGAACTAGAATGAACAGTGCA	TCAATTGGTAAGAGCACCGC	40.9	50.0	57.4	58.6	1090-1117 bp	1.0	65.4
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	AATTGGTAAGAGCACCGCC	45.0	52.6	55.8	58.1	1081-1108 bp	1.0	64.0
psbM-trnD-GUC_p4	GGAACTAGAATGAACAGTGCAG	TCAATTGGTAAGAGCACCGC	45.5	50.0	57.1	58.6	1089-1116 bp	1.0	62.9
psbM-trnD-GUC_p5	GGCAGTAGGAACTAGAATGAACA	TCAATTGGTAAGAGCACCGC	43.5	50.0	57.9	58.6	1096-1123 bp	1.0	61.7