Hotspot

petN-psbM

LSC · 28548-29610 · 1063 bp

Back to Kalanchoe

Pi 0.0343

MarkerSeek score 67.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1063
variable_sites: 107
pi: 0.0343
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CGTGGGCTGCTTTAATGGTA	CCTTCGGGTCCAAAGAAACA	50.0	50.0	58.3	58.0	1543-1809 bp	0.857143	82.5
petN-psbM_p2	TAGGCTATCCCCCAATTGGT	CCTTCGGGTCCAAAGAAACA	50.0	50.0	57.8	58.0	1964-2228 bp	0.857143	82.2
petN-psbM_p3	TAGGCTATCCCCCAATTGGT	TTTGTCCTTCGGGTCCAAAG	50.0	50.0	57.8	58.0	1969-2233 bp	0.714286	75.8
petN-psbM_p4	ATTGAATGAAGCCGGACAGG	TTTGTCCTTCGGGTCCAAAG	50.0	50.0	58.2	58.0	2164-2407 bp	0.714286	75.7
petN-psbM_p5	ATTGAATGAAGCCGGACAGG	CCTTCGGGTCCAAAGAAACA	50.0	50.0	58.2	58.0	2159-2402 bp	0.571429	69.4