Hotspot

rps2-rpoC2

LSC · 15875-16110 · 236 bp

Back to Kalanchoe

Pi 0.0388

MarkerSeek score 66.30

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 236
variable_sites: 28
pi: 0.0388
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	GCCATTCTCGGATTCCACTT	GTTGAGAGTTGGAACGAGCA	50.0	50.0	58.0	58.1	985-1012 bp	1.0	85.7
rps2-rpoC2_p2	GGCTACTGAATCAGCTGCTT	GTTGAGAGTTGGAACGAGCA	50.0	50.0	58.0	58.1	1145-1172 bp	1.0	85.1
rps2-rpoC2_p3	GCCATTCTCGGATTCCACTT	CAGAGCAGATTCAGCCCTTT	50.0	50.0	58.0	57.9	352-358 bp	0.357143	57.7
rps2-rpoC2_p4	AAGGTGCCATTCTCGGATTC	CAGAGCAGATTCAGCCCTTT	50.0	50.0	58.0	57.9	357-363 bp	0.357143	57.6
rps2-rpoC2_p5	GGCTACTGAATCAGCTGCTT	CAGAGCAGATTCAGCCCTTT	50.0	50.0	58.0	57.9	512-518 bp	0.357143	56.7