Hotspot

trnG-GCC-trnR-UCU

LSC · 9735-9972 · 238 bp

Back to Kalanchoe

Pi 0.0334

MarkerSeek score 66.10

Primer available yes

Label trnG-GCC-trnR-UCU

Feature Metadata

label_name: trnG-GCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 238
variable_sites: 25
pi: 0.0334
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnR-UCU_p1	TAGCCTTCCAAGCTACCGAT	ATGGCAATAAACTGCGTCCA	50.0	45.0	58.2	58.2	339-367 bp	1.0	86.9
trnG-GCC-trnR-UCU_p2	TCCTGAGTTGACGTCGACTA	ATGGCAATAAACTGCGTCCA	50.0	45.0	57.8	58.2	366-394 bp	1.0	86.8
trnG-GCC-trnR-UCU_p3	AATCCTGAGTTGACGTCGAC	ATGGCAATAAACTGCGTCCA	50.0	45.0	57.7	58.2	368-396 bp	1.0	85.4
trnG-GCC-trnR-UCU_p4	TCCTGAGTTGACGTCGACTA	TGGCAATAAACTGCGTCCAA	50.0	45.0	57.8	58.4	365-393 bp	1.0	85.0
trnG-GCC-trnR-UCU_p5	TGACGTCGACTATAACCCCT	ATGGCAATAAACTGCGTCCA	50.0	45.0	57.6	58.2	358-386 bp	1.0	84.9