Hotspot

trnS-GCU-trnG-GCC

LSC · 8363-8964 · 602 bp

Back to Kalanchoe

Pi 0.0346
MarkerSeek score 75.50
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
602
variable_sites
68
pi
0.0346
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 629-668 bp 1.0 59.9
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 628-667 bp 1.0 59.9
trnS-GCU-trnG-GCC_p3 CAATCCGACGCTTTAGTCCA ACGAATCTCACTTTTACCACT 50.0 38.1 58.0 54.9 643-682 bp 1.0 59.1
trnS-GCU-trnG-GCC_p4 AATCCGACGCTTTAGTCCAC ACGAATCTCACTTTTACCACT 50.0 38.1 58.0 54.9 642-681 bp 1.0 59.1
trnS-GCU-trnG-GCC_p5 CAATCCGACGCTTTAGTCCA TTACCACTAAACTATACCCGC 50.0 42.9 58.0 54.9 630-669 bp 1.0 58.8