Hotspot

rps16-trnQ-UUG

LSC · 5927-7022 · 1096 bp

Back to Kalanchoe

Pi 0.0397

MarkerSeek score 78.50

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1096
variable_sites: 132
pi: 0.0397
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1169-1560 bp	1.0	90.3
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1180-1571 bp	1.0	89.8
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	CTATGCGGAGGTTCGAATCC	45.0	55.0	58.1	58.2	1176-1567 bp	1.0	89.2
rps16-trnQ-UUG_p4	TCATGTCCTTCAAGTCGCAC	CTATGCGGAGGTTCGAATCC	50.0	55.0	58.2	58.2	1187-1578 bp	1.0	88.6
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	TATGCGGAGGTTCGAATCCT	45.0	50.0	58.1	58.6	1175-1566 bp	1.0	86.2