Hotspot

rpl16

LSC · 83916-85312 · 1397 bp

Back to Kageneckia

Pi 0.0067

MarkerSeek score 50.90

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1397
variable_sites: 14
pi: 0.0067
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GATCAATAACAACCGCAGCG	AACAGGCAGGTACAAAAGGG	50.0	50.0	57.9	58.0	2169-2170 bp	1.0	81.0
rpl16_p2	CCTTTCGTTTGTTTCTACATTC	AAAGAACAATTCTATAAGGTTGAA	36.4	25.0	54.0	52.6	1471-1476 bp	1.0	41.6
rpl16_p3	CCTTTCGTTTGTTTCTACATTC	AAGAACAATTCTATAAGGTTGAAT	36.4	25.0	54.0	52.4	1470-1475 bp	1.0	41.6
rpl16_p4	CCTTTCGTTTGTTTCTACATT	AAAGAACAATTCTATAAGGTTGAA	33.3	25.0	52.7	52.6	1471-1476 bp	1.0	41.6
rpl16_p5	CCTTTCGTTTGTTTCTACATT	AAGAACAATTCTATAAGGTTGAAT	33.3	25.0	52.7	52.4	1470-1475 bp	1.0	41.6