Hotspot

ndhD

SSC · 119670-121172 · 1503 bp

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Pi 0.0071

MarkerSeek score 51.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 16
pi: 0.0071
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CTGCAATTGTGGCCTCAATG	AGAGGACTGTGTCGGTTGTA	50.0	50.0	58.0	58.0	2116 bp	1.0	80.6
ndhD_p2	CTGCAATTGTGGCCTCAATG	CTATTGGCCCGGCTATTGTT	50.0	50.0	58.0	58.0	2578 bp	1.0	80.6
ndhD_p3	CTGCAATTGTGGCCTCAATG	TCTGCTCCAAGAACAGAGGA	50.0	50.0	58.0	58.0	2130 bp	1.0	80.5
ndhD_p4	CTGCAATTGTGGCCTCAATG	GGGACGGGTGTAAAGCTAAG	50.0	55.0	58.0	58.0	2159 bp	1.0	80.5
ndhD_p5	CTGCAATTGTGGCCTCAATG	CTTTACCGACAAAAACCCGC	50.0	50.0	58.0	58.0	1960 bp	1.0	80.3