Hotspot

trnH-GUG-psbA

LSC · 162-434 · 273 bp

Back to Kageneckia

Pi 0.0113

MarkerSeek score 56.00

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 273
variable_sites: 4
pi: 0.0113
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	ATATTATGGGCGAACGACGG	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	424-451 bp	1.0	83.9
trnH-GUG-psbA_p2	ATATTATGGGCGAACGACGG	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	374-401 bp	1.0	83.7
trnH-GUG-psbA_p3	ATATTATGGGCGAACGACGG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	377-404 bp	1.0	83.6
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	55.0	50.0	58.1	58.0	363-390 bp	1.0	83.6
trnH-GUG-psbA_p5	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	50.0	50.0	57.9	58.0	377-404 bp	1.0	83.5