Hotspot

trnH-GUG-rpl2

IRb · 88662-88707 · 46 bp

Back to Kaempferia

Pi 0.0543
MarkerSeek score 79.80
Primer available yes
Label trnH-GUG-rpl2

Feature Metadata

label_name
trnH-GUG-rpl2
feature_type
igs
region
IRb
length_bp
46
variable_sites
5
pi
0.0543
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-rpl2_p1 TGGATCAAGGCAGTGGATTG GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 182-184 bp 1.0 80.9
trnH-GUG-rpl2_p2 GGATCAAGGCAGTGGATTGT GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 181-183 bp 1.0 80.9
trnH-GUG-rpl2_p3 GTGGATCAAGGCAGTGGATT GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 183-185 bp 1.0 80.9
trnH-GUG-rpl2_p4 CGGATGTAGCCAAGTGGATC GTTATCCTGCGCTTGGAAGA 55.0 50.0 58.1 58.0 196-198 bp 1.0 80.8
trnH-GUG-rpl2_p5 TAGCTAGAAAAGGGGCGGAT GTTATCCTGCGCTTGGAAGA 50.0 50.0 58.2 58.0 211-213 bp 1.0 80.1