Hotspot

rpl22-rps19

IRb · 87907-88156 · 250 bp

Back to Kaempferia

Pi 0.029

MarkerSeek score 63.60

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 250
variable_sites: 13
pi: 0.029
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GCCCTTTGAAAACAGACAT	TACGAGACACGCAAGAAACG	42.1	50.0	53.7	58.6	292-331 bp	1.0	47.3
rpl22-rps19_p2	GCCCTTTGAAAACAGACAT	GTGGGTCACAAATTGGGAGA	42.1	50.0	53.7	57.7	333-372 bp	1.0	47.0
rpl22-rps19_p3	GCCCTTTGAAAACAGACAT	ATGGTGGGTCACAAATTGGG	42.1	50.0	53.7	58.4	336-375 bp	1.0	46.9
rpl22-rps19_p4	GCCCTTTGAAAACAGACAT	GATCGTATGGTGGGTCACAA	42.1	50.0	53.7	57.3	342-381 bp	1.0	46.9
rpl22-rps19_p5	GCCCTTTGAAAACAGACAT	TAACGGATCGTATGGTGGGT	42.1	50.0	53.7	58.2	347-386 bp	1.0	46.8