Pi
0.0231
MarkerSeek score
63.40
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1148-1174 bp | 1.0 | 85.9 |
| trnT-UGU-trnL-UAA_p2 | GAGTCGAACCGATGACCATC | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 58.2 | 58.0 | 1179-1205 bp | 1.0 | 85.6 |
| trnT-UGU-trnL-UAA_p3 | AATTGTCGCTCTGTCAGACC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.9 | 58.0 | 1823-1834 bp | 1.0 | 85.4 |
| trnT-UGU-trnL-UAA_p4 | AATTGTCGCTCTGTCAGACC | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.9 | 58.1 | 1831-1842 bp | 1.0 | 84.8 |
| trnT-UGU-trnL-UAA_p5 | TGTCGCTCTGTCAGACCATA | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.2 | 58.0 | 1820-1831 bp | 1.0 | 84.7 |