Hotspot

trnfM-CAU-rps14

LSC · 39248-39404 · 157 bp

Back to Kaempferia

Pi 0.0372

MarkerSeek score 69.50

Primer available yes

Label trnfM-CAU-rps14

Feature Metadata

label_name: trnfM-CAU-rps14
feature_type: igs
region: LSC
length_bp: 157
variable_sites: 11
pi: 0.0372
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-rps14_p1	AAGGTTATGAGCCTTGCGAG	TTACCGGGCGCAATAAGATC	50.0	50.0	58.0	58.1	230 bp	1.0	82.6
trnfM-CAU-rps14_p2	TTTGAACCCGTGACCTCAAG	TTACCGGGCGCAATAAGATC	50.0	50.0	58.0	58.1	247 bp	1.0	82.2
trnfM-CAU-rps14_p3	ACAGGATTTGAACCCGTGAC	TTACCGGGCGCAATAAGATC	50.0	50.0	58.1	58.1	253 bp	1.0	81.6
trnfM-CAU-rps14_p4	AAGGTTATGAGCCTTGCGAG	ATGTTTATTACCGGGCGCAA	50.0	45.0	58.0	58.2	237 bp	1.0	81.5
trnfM-CAU-rps14_p5	AAGGTTATGAGCCTTGCGAG	TGTTTATTACCGGGCGCAAT	50.0	45.0	58.0	58.2	236 bp	1.0	81.5