Hotspot

trnD-GUC-trnY-GUA

LSC · 32579-33014 · 436 bp

Back to Kaempferia

Pi 0.0149

MarkerSeek score 61.00

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 436
variable_sites: 12
pi: 0.0149
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	AATTCTTCCTGGGTCGATGC	50.0	50.0	58.5	58.0	545-575 bp	1.0	79.8
trnD-GUC-trnY-GUA_p2	ATCCTAGTTCGGGACTGACG	AATTCTTCCTGGGTCGATGC	55.0	50.0	58.6	58.0	598-628 bp	1.0	78.4
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	512-542 bp	1.0	77.6
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	511-541 bp	1.0	77.4
trnD-GUC-trnY-GUA_p5	ATCCTAGTTCGGGACTGACG	GGGGACGGACTGTAAATTCG	55.0	55.0	58.6	58.4	565-595 bp	1.0	76.2