Hotspot

rps16-trnQ-UUG

LSC · 5929-7381 · 1453 bp

Back to Kaempferia

Pi 0.016
MarkerSeek score 64.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1453
variable_sites
40
pi
0.016
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.90
markerseek_score
64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CAGCCGATCATGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 839-1545 bp 1.0 85.6
rps16-trnQ-UUG_p2 GCCGATCATGTCCTTCAAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 837-1543 bp 1.0 85.5
rps16-trnQ-UUG_p3 AATCCACAGCCGATCATGTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 845-1551 bp 1.0 85.4
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 821-1527 bp 1.0 84.7
rps16-trnQ-UUG_p5 CAGCCGATCATGTCCTTCAA CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.0 57.8 843-1549 bp 1.0 84.5