Hotspot

matK-trnK-UUU

LSC · 3314-4140 · 827 bp

Back to Kaempferia

Pi 0.00726

MarkerSeek score 61.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 827
variable_sites: 10
pi: 0.00726
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGAAACTGTTGTTGCCGAGA	TGAAGGGTTGGGTTGCTAAC	45.0	50.0	57.7	58.0	929-951 bp	0.75	69.1
matK-trnK-UUU_p2	AGAAACTGTTGTTGCCGAGA	TTTTGAAGGGTTGGGTTGCT	45.0	45.0	57.7	58.1	932-954 bp	0.75	68.2
matK-trnK-UUU_p3	AGAAACTGTTGTTGCCGAGA	AACGGTAGAGTACTCGGCTT	45.0	50.0	57.7	58.2	907-929 bp	0.75	67.9
matK-trnK-UUU_p4	AGAAACTGTTGTTGCCGAGA	ACGGTAGAGTACTCGGCTTT	45.0	50.0	57.7	58.2	906-928 bp	0.75	67.9
matK-trnK-UUU_p5	AACTGTTGTTGCCGAGATCT	TGAAGGGTTGGGTTGCTAAC	45.0	50.0	57.4	58.0	926-948 bp	0.75	67.3