Hotspot

ndhF

Genome · 111288-113522 · 2235 bp

Back to Kadsura

Pi 0.0135

MarkerSeek score 62.10

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: Genome
length_bp: 2235
variable_sites: 62
pi: 0.0135
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AACTCCTGAAGGCCTGGATA	AGCTGGTGAATCGGAAACAA	50.0	45.0	57.7	57.7	2323-2336 bp	0.833333	70.9
ndhF_p2	ACTCCTGAAGGCCTGGATAA	AGCTGGTGAATCGGAAACAA	50.0	45.0	57.7	57.7	2322-2335 bp	0.833333	70.9
ndhF_p3	AAGAAACTCCTGAAGGCCTG	AGCTGGTGAATCGGAAACAA	50.0	45.0	57.4	57.7	2327-2340 bp	0.833333	68.4
ndhF_p4	AAACTCCTGAAGGCCTGGAT	AGCTGGTGAATCGGAAACAA	50.0	45.0	58.6	57.7	2324-2337 bp	0.833333	68.0
ndhF_p5	AACTCCTGAAGGCCTGGAT	AGCTGGTGAATCGGAAACAA	52.6	45.0	57.9	57.7	2323-2336 bp	0.833333	64.1