Hotspot

ndhD

Genome · 116843-118363 · 1521 bp

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Pi 0.00767

MarkerSeek score 49.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: Genome
length_bp: 1521
variable_sites: 25
pi: 0.00767
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GCTAATGAGGCATGGGGATC	GCTATTGGGCCAGCTATTGT	55.0	50.0	58.2	58.0	2561-2766 bp	1.0	84.9
ndhD_p2	GGGAGCAGTATGGGCTAATG	GCTATTGGGCCAGCTATTGT	55.0	50.0	57.8	58.0	2574-2779 bp	1.0	84.7
ndhD_p3	GCTAATGAGGCATGGGGATC	CTGCTCCAAGAACAGAGGAC	55.0	55.0	58.2	57.9	2087-2294 bp	1.0	84.6
ndhD_p4	GTGTCATTGGCCTAGGGTTT	GCTATTGGGCCAGCTATTGT	50.0	50.0	57.8	58.0	2620-2825 bp	1.0	84.5
ndhD_p5	GGGAGCAGTATGGGCTAATG	CTGCTCCAAGAACAGAGGAC	55.0	55.0	57.8	57.9	2100-2307 bp	1.0	84.4